Agroinoculation of Geminiviral Infectious Clones into Plants
摘要
Most geminiviruses are not transmitted by mechanical inoculation. Therefore, pathogenicity and plant–pathogen interaction studies rely on agroinoculation using infectious clones, which involves cloning the geminiviral genome in a binary vector (see previous chapter for details). A suspension containing the infectious clone inserted into Agrobacterium tumefaciens cells is then inoculated into plants, i.e., agroinoculated. Below is a simple protocol for agroinoculation of an infectious geminivirus clone into plants.