The Split-Luciferase Complementation Assay to Detect and Quantify Protein–Protein Interactions in Planta

Protein–protein interactions play a critical role in plant viral infection and defense responses against pathogens. This protocol provides a detailed and reliable methodology for investigating protein–protein interactions using a luciferase-based complementation assay that includes easy luminescence-based normalization within a single plate. The protocol includes step-by-step procedures, reagent lists, and considerations for data interpretation, ensuring robust and reproducible results. By following this protocol, researchers can advance on understanding of the crucial role of protein–protein interactions in plant viral infection and defense responses to other pathogen attacks.