Chromatin Immunoprecipitation Sequencing (ChIP-Seq) Assay in Food Allergy Research
摘要
Chromatin immunoprecipitation (ChIP) analysis followed by genome-wide sequencing represents the most efficient method for studying the complex interactions between nuclear proteins and their associated genomic DNA. This chapter aims to present a ChIP-Sequencing (ChIP-Seq) analysis protocol used for the investigation of different epigenetic modifications in diseases such as food allergy. Starting by treating cells with cross-linking reagents such as formaldehyde and ending up with profound bioinformatic analysis have made it plausible to understand how transcription factors and histone modifications localize and exert their effects on their associated genes.