Components Subcellular Localization: Identification of Lipoproteins Using Globomycin and Radioactive Palmitate

Bacterial lipoproteins are characterized by fatty acids, derived from membrane phospholipids, which are covalently attached to their amino terminus via posttranslational modification in the cytoplasmic membrane. Here, I describe the detection of one of the intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using 3H-palmitate labeling and inhibition of signal peptidase II (Lsp) by globomycin and detection by fluorography.