Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages

实验方案
发表日期：

2024年

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Clara Jana-Lui Busch,
Sethuraman Subramanian,
Javier Linares,
Jérémy Favret,
Ridzky Anis Advent Yuda,
Michael H. Sieweke

摘要

Alveolar macrophages (AM) are resident macrophages of the lung and play important roles in the maintenance of tissue homeostasis as well as host defense. Here, we describe how they can be harvested from murine lungs, expanded in vitro, and transduced with lentiviral vectors.