Fluorescence Microscopy of Nanochannel-Confined DNA

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发表日期：

2024年

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Sriram KK,
Fredrik Persson,
Joachim Fritzsche,
Jason P. Beech,
Jonas O. Tegenfeldt,
Fredrik Westerlund

摘要

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.