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Skin epidermal permeability is a critical barrier function, and its disruption is implicated in various dermatological conditions and in secondary lymphedema, leading to increased fluid loss and dermal complications. Many techniques exist to assess this permeability. However, these methods frequently require specialized instruments or lack the spatial resolution needed to identify specific cellular layers involved in barrier dysfunction. Here, we describe a step-by-step protocol for an immunofluorescence-based dye penetration assay that provides high spatial resolution for visualizing the permeation of fluorescent probes through specific epidermal strata in a mouse lymphedema model.

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Assessing Epidermal Permeability in Lymphedema Skin with Sulfo-NHS-Biotin Immunofluorescent Penetration Assay

  • 实验方案

  • 发表日期：

    2026年

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  • Xizhao Chen,
  • Jung Eun Baik,
  • Jinyeon Shin,
  • Annica R. Stull-Lane,
  • Gopika Ashokan,
  • Sarit Pal,
  • Geoffrey E. Hespe,
  • Babak J. Mehrara,
  • Raghu P. Kataru

摘要

Skin epidermal permeability is a critical barrier function, and its disruption is implicated in various dermatological conditions and in secondary lymphedema, leading to increased fluid loss and dermal complications. Many techniques exist to assess this permeability. However, these methods frequently require specialized instruments or lack the spatial resolution needed to identify specific cellular layers involved in barrier dysfunction. Here, we describe a step-by-step protocol for an immunofluorescence-based dye penetration assay that provides high spatial resolution for visualizing the permeation of fluorescent probes through specific epidermal strata in a mouse lymphedema model.