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Effective epidermal cell turnover is essential for the formation and maintenance of the epidermis. Dysregulation of keratinocyte turnover impedes skin barrier integrity and contributes to disease pathophysiology. Full-thickness human skin equivalents (FT-HSE) offer a physiologically relevant in vitro platform for studying skin biology, providing valuable insights into the epidermal response to both endogenous and exogenous stimuli. This chapter describes immunofluorescent staining of Ki67 and the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay for assessing cellular proliferation and apoptosis, thereby providing insights into keratinocyte turnover within FT-HSEs.

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Biometric Quantification of Keratinocyte Turnover Using Bioengineered Human Skin Equivalents

  • 实验方案

  • 发表日期：

    2025年

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  • Georgia Abraham,
  • Julia Maczkowska,
  • Rachel Gilmore,
  • Nicole Barrett,
  • Jessica Simpson,
  • Paola De Los Santos Gomez,
  • Kirsty Goncalves,
  • Victoria Maltman,
  • Stefan Przyborski

摘要

Effective epidermal cell turnover is essential for the formation and maintenance of the epidermis. Dysregulation of keratinocyte turnover impedes skin barrier integrity and contributes to disease pathophysiology. Full-thickness human skin equivalents (FT-HSE) offer a physiologically relevant in vitro platform for studying skin biology, providing valuable insights into the epidermal response to both endogenous and exogenous stimuli. This chapter describes immunofluorescent staining of Ki67 and the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay for assessing cellular proliferation and apoptosis, thereby providing insights into keratinocyte turnover within FT-HSEs.