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Determining the melanogenic capacity of basal melanocytes is vital when studying skin pigmentation and related disorders. Human skin equivalents (HSEs) can be used as a valuable tool to study pigmentation in vitro, providing in-depth analysis and characterization of molecular mechanisms associated with melanogenesis. Here, we describe a method to both visualize and quantify basal melanocytes within the epidermis of a pigmented HSE. By using an image analysis pipeline, we can determine how melanocyte density changes in response to treatment and how melanogenic activity is altered using immunofluorescent staining techniques.

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Visualization and Quantification of Basal Melanocytes Within a 3D Bioengineered Skin Equivalent

  • 实验方案

  • 发表日期：

    2025年

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  • Nicole Barrett,
  • Mia Lindsay,
  • Paola De Los Santos Gomez,
  • Jessica Simpson,
  • Victoria Maltman,
  • Kirsty Goncalves,
  • Stefan Przyborski

摘要

Determining the melanogenic capacity of basal melanocytes is vital when studying skin pigmentation and related disorders. Human skin equivalents (HSEs) can be used as a valuable tool to study pigmentation in vitro, providing in-depth analysis and characterization of molecular mechanisms associated with melanogenesis. Here, we describe a method to both visualize and quantify basal melanocytes within the epidermis of a pigmented HSE. By using an image analysis pipeline, we can determine how melanocyte density changes in response to treatment and how melanogenic activity is altered using immunofluorescent staining techniques.