Scholarlink

Human embryogenesis has been problematic to study due to technical and ethical issues. Recently, a human embryo model generated from pluripotent stem cells (PSCs) to mimic human embryogenesis, which has attracted attention as an invaluable tool for studying human embryonic development. We have successfully developed a method to efficiently induce the differentiation of naïve human PSCs, which correspond to preimplantation epiblasts, into extraembryonic cells of the blastocyst. Furthermore, by combining these cells, we developed a novel nonintegrated human embryo model called “bilaminoid” that reproduces development from preimplantation to peri-gastrulation stages. Here, we describe a detailed protocol for the bilaminoid formation.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Generation of the Nonintegrated Human Bilaminar Embryo Model (Bilaminoid) from Naïve Pluripotent Stem Cells

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Takumi Okubo,
  • Yasuhiro Takashima

摘要

Human embryogenesis has been problematic to study due to technical and ethical issues. Recently, a human embryo model generated from pluripotent stem cells (PSCs) to mimic human embryogenesis, which has attracted attention as an invaluable tool for studying human embryonic development. We have successfully developed a method to efficiently induce the differentiation of naïve human PSCs, which correspond to preimplantation epiblasts, into extraembryonic cells of the blastocyst. Furthermore, by combining these cells, we developed a novel nonintegrated human embryo model called “bilaminoid” that reproduces development from preimplantation to peri-gastrulation stages. Here, we describe a detailed protocol for the bilaminoid formation.