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During the peri-implantation stage in primate embryos, the epiblast coexists with amniotic epithelium, suggesting a pivotal role for this tissue in embryogenesis. This interaction has attracted considerable interest in developmental biology and regenerative medicine due to its potential influence on embryonic patterning and tissue specification. In this protocol, we examined whether co-culturing human amniotic epithelial cells (hAEC) with human embryonic stem cells (hESC) enhances the latter’s capacity for differentiation toward human primordial germ cell-like cells (hPGCLC).

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Interaction Between Pluripotent Stem Cells and Amniotic Epithelium for the In Vitro Study of Human Embryogenesis

  • 实验方案

  • 发表日期：

    2025年

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  • Daniela Ávila-González,
  • Axel Castro-Abrego,
  • Jonathan Salazar-Alonso,
  • Omar Martínez Alarcon,
  • Alejandro Martinez-Juarez,
  • Anayansi Molina Hernández,
  • Nestor Emmanuel Diaz-Martinez,
  • Wendy Portillo,
  • Nestor Fabián Diaz

摘要

During the peri-implantation stage in primate embryos, the epiblast coexists with amniotic epithelium, suggesting a pivotal role for this tissue in embryogenesis. This interaction has attracted considerable interest in developmental biology and regenerative medicine due to its potential influence on embryonic patterning and tissue specification. In this protocol, we examined whether co-culturing human amniotic epithelial cells (hAEC) with human embryonic stem cells (hESC) enhances the latter’s capacity for differentiation toward human primordial germ cell-like cells (hPGCLC).