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The bone marrow (BM) niche comprises of diverse cell types, each playing a distinct role in hematopoiesis and disease. Single-cell RNA sequencing enables investigation of various cell populations within heterogeneous tissues. In this chapter, we describe the method to isolate BM niche cells from femurs and tibias of mice by fluorescence-activated cell sorting for single-cell RNA sequencing. This method ensures isolation of high-quality BM niche cells with high viability and purity.

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Isolation of Mouse Bone Marrow Niche Cells for Single-Cell Sequencing

  • 实验方案

  • 发表日期：

    2025年

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  • Yaphet Bustos,
  • Adrienne M. Dorrance,
  • Chinmayee Goda

摘要

The bone marrow (BM) niche comprises of diverse cell types, each playing a distinct role in hematopoiesis and disease. Single-cell RNA sequencing enables investigation of various cell populations within heterogeneous tissues. In this chapter, we describe the method to isolate BM niche cells from femurs and tibias of mice by fluorescence-activated cell sorting for single-cell RNA sequencing. This method ensures isolation of high-quality BM niche cells with high viability and purity.