An In Vitro Delayed Scratch Closure Assay with Features of Chronic Wounds

A scratch assay is a simple wound healing model for assessing cell migration in which a confluent cell layer is scratched to create a scratch zone (“wound”) that cells migrate into. Here, we describe a scratch assay exhibiting delayed closure and increased inflammation, which more closely resemble chronic wounds. This delayed scratch closure assay is more relevant for screening compounds and studying delayed wound healing compared with traditional scratch wound assays that more closely resemble acute wounds.