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Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL

  • Tiam Heydari,
  • Peter W. Zandstra

摘要

IQCELL is a platform that infers Boolean gene regulatory networks from single-cell RNA sequencing data. Boolean networks can be simulated under normal and perturbed conditions. In this chapter, we provide a detailed guideline for implementing IQCELL from a raw dataset. The steps include processing data, inferring informative genes, inferring gene regulatory network, and simulating the resulted network under normal and perturbed conditions.