<p class="MsoNormal" style="line-height: normal; mso-layout-grid-align: none; text-autospace: none;"><span lang="DE-CH" style="font-size: 12.0pt; font-family: 'Times New Roman',serif;">This second volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. Chapters describe the isolation, culture of diverse skin cells, skin models, and managing these cells within co-culture systems. </span><span style="font-size: 12.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: Aptos; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US;">Written in the highly successful <em>Methods in Molecular Biology </em>series format, the chapters include brief introductions to<em> </em>the material, lists of necessary materials and reagents, step-by-step, readily<em> </em>reproducible laboratory protocols, and a Notes section which highlights<em> </em>tips on troubleshooting and avoiding known pitfalls.</span></p><p class="MsoNormal" style="line-height: normal; mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 12.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: Aptos; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US;">&#xa0;</span><span style="font-size: 12.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: Aptos; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US;">Authoritative and cutting-edge, </span><em><span lang="DE-CH" style="font-size: 12.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: Aptos; mso-fareast-theme-font: minor-latin; mso-fareast-language: EN-US;">Skin Tissue Engineering</span></em><em><span style="font-size: 12.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: Aptos; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US;">: Methods and Protocols, Second Edition </span></em><span style="font-size: 12.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: Aptos; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US;">aims to be comprehensive guide for<em> </em>researchers in the field.</span></p>

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Skin Tissue Engineering

摘要

This second volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. Chapters describe the isolation, culture of diverse skin cells, skin models, and managing these cells within co-culture systems. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Skin Tissue Engineering: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.