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<p>This second edition volume expands&#xa0;on the previous edition with new chapters and updated discussions on the latest advancements in the development and practice of metabolic&#xa0;phenotyping. The chapters in this book cover topics such as quality control in untargeted metabolic phenotyping; implementation of quality assurances processes; careful bio- and chemoinformatic data analysis; using reversed-phase and ion-pair LC-MS; quantitative lipid analysis using supercritical fluid chromatography (SFC-MS)&#xa0;and the&#xa0;targeted&#xa0; determination of metanephrines in urine via LC-MS/MS. Written in the highly successful&#xa0;<em>Methods in Molecular Biology</em>&#xa0;series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on&#xa0;troubleshooting<strong>&#xa0;</strong>and avoiding known pitfalls.<br><br>Thorough and comprehensive,&#xa0;<em>Metabolic Profiling: Methods and Protocols, Second Edition&#xa0;</em>is a valuable resource for all researchers who are interested in incorporating best practices in metabolic phenotyping and learning more about this&#xa0;rapidly<strong>&#xa0;</strong>advancing field.</br></br></p>

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Metabolic Profiling

  • 书籍

  • 发表日期：

    2025年

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摘要

This second edition volume expands on the previous edition with new chapters and updated discussions on the latest advancements in the development and practice of metabolic phenotyping. The chapters in this book cover topics such as quality control in untargeted metabolic phenotyping; implementation of quality assurances processes; careful bio- and chemoinformatic data analysis; using reversed-phase and ion-pair LC-MS; quantitative lipid analysis using supercritical fluid chromatography (SFC-MS) and the targeted  determination of metanephrines in urine via LC-MS/MS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and comprehensive, Metabolic Profiling: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in incorporating best practices in metabolic phenotyping and learning more about this rapidly advancing field.