Scholarlink

<p>This study aimed to investigate the effects of various growth conditions and nutritional stress on the rate of cell development, auto and co-aggregation abilities, cell surface hydrophobicity, adherence to HT-29 cells, and changes in cellular fatty acid composition of <i>Limosilactobacillus fermentum</i> JNU532 (JNU532). The strain JNU532 was exposed to different stressors, including temperature variations (37°C and 42°C), salt (3% NaCl), acid (pH 4), and alkali (pH 8). Following exposure to temperature stress at 42°C, the strain demonstrated the highest levels of auto- and co-aggregation, but the lowest levels of hydrophobicity and adhesion abilities. Under acidic conditions, the strain’s development remained stable, but its auto and co-aggregation abilities were enhanced compared to those grown at a temperature of 37°C and pH 6.5. Moreover, JNU532 grown at pH 4 showed relatively high cell surface hydrophobicity and adhesion abilities. However, the strain exhibited the greatest adhesion capacity when subjected to alkaline stress compared to the control and other stress groups. Gas chromatography was used to isolate and quantitatively assess the levels of intracellular oleic acid and its post-synthetic derivatives, including cis-9,10-methyleneoctadecanoic acid (C19:0 cyclo ω8c) and C19:0 cyclo ω10c/19ω6. The JNU532 exhibited the highest amount of oleic acid during temperature stress at 42°C. Furthermore, JNU532 demonstrated the highest yield of the oleic acid cyclopropanation product C19:0 cyclo ω10c/19ω6 under acidic conditions (pH 4). Therefore, this study the application of JNU 532 as a probiotic in the food and milk industry has laid a theoretical foundation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Environmental Stress on Limosilactobacillus fermentum JNU532’s Cellular Characteristics and Fatty Acid Profile

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年5月1日

查看全文
  • Ziyao Meng,
  • Sejong Oh

摘要

This study aimed to investigate the effects of various growth conditions and nutritional stress on the rate of cell development, auto and co-aggregation abilities, cell surface hydrophobicity, adherence to HT-29 cells, and changes in cellular fatty acid composition of Limosilactobacillus fermentum JNU532 (JNU532). The strain JNU532 was exposed to different stressors, including temperature variations (37°C and 42°C), salt (3% NaCl), acid (pH 4), and alkali (pH 8). Following exposure to temperature stress at 42°C, the strain demonstrated the highest levels of auto- and co-aggregation, but the lowest levels of hydrophobicity and adhesion abilities. Under acidic conditions, the strain’s development remained stable, but its auto and co-aggregation abilities were enhanced compared to those grown at a temperature of 37°C and pH 6.5. Moreover, JNU532 grown at pH 4 showed relatively high cell surface hydrophobicity and adhesion abilities. However, the strain exhibited the greatest adhesion capacity when subjected to alkaline stress compared to the control and other stress groups. Gas chromatography was used to isolate and quantitatively assess the levels of intracellular oleic acid and its post-synthetic derivatives, including cis-9,10-methyleneoctadecanoic acid (C19:0 cyclo ω8c) and C19:0 cyclo ω10c/19ω6. The JNU532 exhibited the highest amount of oleic acid during temperature stress at 42°C. Furthermore, JNU532 demonstrated the highest yield of the oleic acid cyclopropanation product C19:0 cyclo ω10c/19ω6 under acidic conditions (pH 4). Therefore, this study the application of JNU 532 as a probiotic in the food and milk industry has laid a theoretical foundation.