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Abstract <p>A nutrigenomic analysis of the antioxidant and stress-protective properties of biologically active compounds of plant origin with different functional properties was performed to subsequently develop a phytogenic compound to improve production performance of broiler chickens and their resistance to oxidative stress. The <i>Smena 9</i> cross broiler chickens (<i>n</i> = 36) were split into control and experimental groups with the balanced group-analogue method at the training poultry production house, Russian State Agricultural University–Moscow Timiryazev Agricultural Academy, which were provided a diet added with different phytogenic bioactive compounds, including common-chicory inulin, St. John’s wort flavonoids, leuzea carthamoides, ecdysterone, and creeping thyme flavonoids and tannins. The experiment had a duration of 35 days (March–April 2024). Liver samples were collected from four chickens of an average live weight both at the age of 26 days to study the expression of genes associated with antioxidant protection and at the age of 32 days to perform histological examination. The <i>SOD1</i> and <i>PRXD6</i> gene expression was analyzed with the 2<sup>−ΔΔCT</sup> method, involving total RNA isolation followed by reverse transcription to generate cDNA, and the RT-PCR technique with control gene <i>ACTB</i>. Histologic scoring indices were determined with microscopy, measuring the hepatocyte length. It was ascertained that the use of chicory and St. John’s wort extracts standardized for inulin and flavonoids, respectively, contributed to the transcriptional activity of the <i>SOD1</i> key antioxidant vitagen, increased 4.47 (<i>p</i> ≤ 0.05) and 3.53 (<i>p</i> ≤ 0.01) times their reference values, respectively, to ensure 100% livestock safety. In case of the use of the St. John’s wort extract containing flavonoids, increases in the absolute and relative weight of the liver (by 26.1 and 22.8%, respectively), the size of hepatocytes (by 69.4%), and their number per unit area was revealed, which is regarded as a factor for activation of the liver antioxidant functions, proven by the results of a transcriptome analysis.</p>

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Antioxidant Gene Expression in Broiler Chickens under Effects of Plant Biologically Active Compounds

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • M. I. Selionova,
  • V. I. Trukhachev,
  • A. Yu. Zagarin,
  • N. P. Belyaeva,
  • E. I. Kulikov

摘要

Abstract

A nutrigenomic analysis of the antioxidant and stress-protective properties of biologically active compounds of plant origin with different functional properties was performed to subsequently develop a phytogenic compound to improve production performance of broiler chickens and their resistance to oxidative stress. The Smena 9 cross broiler chickens (n = 36) were split into control and experimental groups with the balanced group-analogue method at the training poultry production house, Russian State Agricultural University–Moscow Timiryazev Agricultural Academy, which were provided a diet added with different phytogenic bioactive compounds, including common-chicory inulin, St. John’s wort flavonoids, leuzea carthamoides, ecdysterone, and creeping thyme flavonoids and tannins. The experiment had a duration of 35 days (March–April 2024). Liver samples were collected from four chickens of an average live weight both at the age of 26 days to study the expression of genes associated with antioxidant protection and at the age of 32 days to perform histological examination. The SOD1 and PRXD6 gene expression was analyzed with the 2−ΔΔCT method, involving total RNA isolation followed by reverse transcription to generate cDNA, and the RT-PCR technique with control gene ACTB. Histologic scoring indices were determined with microscopy, measuring the hepatocyte length. It was ascertained that the use of chicory and St. John’s wort extracts standardized for inulin and flavonoids, respectively, contributed to the transcriptional activity of the SOD1 key antioxidant vitagen, increased 4.47 (p ≤ 0.05) and 3.53 (p ≤ 0.01) times their reference values, respectively, to ensure 100% livestock safety. In case of the use of the St. John’s wort extract containing flavonoids, increases in the absolute and relative weight of the liver (by 26.1 and 22.8%, respectively), the size of hepatocytes (by 69.4%), and their number per unit area was revealed, which is regarded as a factor for activation of the liver antioxidant functions, proven by the results of a transcriptome analysis.