Scholarlink

Abstract <p>One of the main problems faced by embryologists when obtaining native and reconstructed animal embryos in vitro is the lack of highly informative tests for animals’ oocyte quality assessment. The study was conducted to develop the noninvasive biomarkers to assess the quality of <i>Ovis aries</i> donor oocytes. Comparative monitoring of chromatin status parameters (pyknosis, mitosis, and apoptosis) of granulosa cells (GCs) and cumulus cells (CCs), relative to the follicle diameter and the functional status of the contained oocytes, was performed. The oocyte cumulus complexes isolated from follicles (&lt;3, 3–5, and 6–8 mm in diameter) were subjected to the BCB test. In GCs, the parameters characterizing chromatin degeneration (pyknosis and apoptosis) were significantly higher than that in CCs from follicles containing both the full-grown and growing oocytes. The proportion of pycnotic CGs from follicles of &lt;3 mm in diameter with BCB (+) oocytes comprised 7%, which was 4% higher than that for CCs (<i>p</i> &lt; 0.01). With respect to the follicles 3–5 mm in diameter, the proportion reached 10 vs. 3% (<i>p</i> &lt; 0.05). The proportion of pycnotic GCs from the follicles of 6–8 mm in diameter with the BCB (+) oocytes was 8% higher than that for CCs (<i>p</i> &lt; 0.001) in the cumulus cells. A similar trend was observed in a comparative analysis of the level of pyknosis in somatic cells of follicles with growing oocytes. Mitotic activities in the follicle somatic cells tended to decrease with their growth more pronounced in the follicles containing full-grown gametes. The chromatin status of CCs and GCs generally reflects the morpho-functional status of a developing oocyte. Meta-analysis of the obtained data revealed a decrease in proliferative activity and an increase in destructive processes in chromatin of somatic cells in the dynamics of follicle growth to identify differences between these indicators relative to the functional status of an oocyte (growing or full-grown). This allows for regarding them as the preventive noninvasive bioindicators of the quality of donor oocytes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Analysis of Chromatin in Somatic Cells of Sheep Follicles Containing Oocytes in Different Functional Status

  • 期刊论文

  • 发表日期：

    2025年2月1日

查看全文
  • T. I. Kuzmina,
  • A. O. Prituzhalova,
  • D. A. Starikova,
  • N. O. Sokolova

摘要

Abstract

One of the main problems faced by embryologists when obtaining native and reconstructed animal embryos in vitro is the lack of highly informative tests for animals’ oocyte quality assessment. The study was conducted to develop the noninvasive biomarkers to assess the quality of Ovis aries donor oocytes. Comparative monitoring of chromatin status parameters (pyknosis, mitosis, and apoptosis) of granulosa cells (GCs) and cumulus cells (CCs), relative to the follicle diameter and the functional status of the contained oocytes, was performed. The oocyte cumulus complexes isolated from follicles (<3, 3–5, and 6–8 mm in diameter) were subjected to the BCB test. In GCs, the parameters characterizing chromatin degeneration (pyknosis and apoptosis) were significantly higher than that in CCs from follicles containing both the full-grown and growing oocytes. The proportion of pycnotic CGs from follicles of <3 mm in diameter with BCB (+) oocytes comprised 7%, which was 4% higher than that for CCs (p < 0.01). With respect to the follicles 3–5 mm in diameter, the proportion reached 10 vs. 3% (p < 0.05). The proportion of pycnotic GCs from the follicles of 6–8 mm in diameter with the BCB (+) oocytes was 8% higher than that for CCs (p < 0.001) in the cumulus cells. A similar trend was observed in a comparative analysis of the level of pyknosis in somatic cells of follicles with growing oocytes. Mitotic activities in the follicle somatic cells tended to decrease with their growth more pronounced in the follicles containing full-grown gametes. The chromatin status of CCs and GCs generally reflects the morpho-functional status of a developing oocyte. Meta-analysis of the obtained data revealed a decrease in proliferative activity and an increase in destructive processes in chromatin of somatic cells in the dynamics of follicle growth to identify differences between these indicators relative to the functional status of an oocyte (growing or full-grown). This allows for regarding them as the preventive noninvasive bioindicators of the quality of donor oocytes.