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<p>The effect of eight bacterial volatile organic compounds (VOCs) with various structures (ketones, alcohols, terpenes) on the functioning of Quorum Sensing (QS) regulatory systems of gram-negative bacterium <i>Escherichia coli</i> was studied. <i>Lux</i>-reporter strains containing components of the QS systems LuxI/LuxR, RhlI/RhlR, and LasI/LasR were used as biosensors in the work. It has been shown that VOCs can modulate QS functioning by acting in different ways. In a study of the effect of ketones (β-ionone, 2‑butanone, 2-pentanone, and 2-octanone), we showed that the unsaturated ketone β-ionone had a strong suppressive effect on the bioluminescence of the <i>Escherichia coli</i> DH5α/pSB401 strain (LuxI/LuxR type QS system) in the presence of N-acylhomoserine lactone (AHL). (+)-α-Pinene also inhibited the QS systems (Quorum Quenching) of the LuxI/LuxR and LasI/LasR types. None of these VOCs affected the growth of bacterial cultures carrying QS-specific biosensory systems. Whereas limonene and isoamyl alcohol reduced bioluminescence and had a toxic effect on the cells of bacterial strains. 2-Butanone and 2-pentanone ketones increased the level of bioluminescence induction of <i>E. coli</i> JLD271/pAL101 (RhlI/RhlR) in the presence of AHLs. The same effect was observed with the terpene (+)-α-pinene. 2-Octanone and 2-phenylethanol directly reduced bioluminescence both in specific biosensors and in strains with an inactivated receptor protein gene (<i>rhlR</i> or <i>lasR</i>), as well as in <i>E. coli</i> strain MG1655/pXen7 with constitutive transcription of the <i>lux</i> operon, i.e. these compounds directly affected the luciferase reaction.</p>

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The Influence of Bacterial Volatile Organic Compounds on Quorum Sensing Regulation

  • 期刊论文

  • 发表日期：

    2025年9月1日

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  • D. E. Sidorova,
  • V. A. Plyuta,
  • E. N. Vagner,
  • O. A. Koksharova,
  • I. A. Khmel

摘要

The effect of eight bacterial volatile organic compounds (VOCs) with various structures (ketones, alcohols, terpenes) on the functioning of Quorum Sensing (QS) regulatory systems of gram-negative bacterium Escherichia coli was studied. Lux-reporter strains containing components of the QS systems LuxI/LuxR, RhlI/RhlR, and LasI/LasR were used as biosensors in the work. It has been shown that VOCs can modulate QS functioning by acting in different ways. In a study of the effect of ketones (β-ionone, 2‑butanone, 2-pentanone, and 2-octanone), we showed that the unsaturated ketone β-ionone had a strong suppressive effect on the bioluminescence of the Escherichia coli DH5α/pSB401 strain (LuxI/LuxR type QS system) in the presence of N-acylhomoserine lactone (AHL). (+)-α-Pinene also inhibited the QS systems (Quorum Quenching) of the LuxI/LuxR and LasI/LasR types. None of these VOCs affected the growth of bacterial cultures carrying QS-specific biosensory systems. Whereas limonene and isoamyl alcohol reduced bioluminescence and had a toxic effect on the cells of bacterial strains. 2-Butanone and 2-pentanone ketones increased the level of bioluminescence induction of E. coli JLD271/pAL101 (RhlI/RhlR) in the presence of AHLs. The same effect was observed with the terpene (+)-α-pinene. 2-Octanone and 2-phenylethanol directly reduced bioluminescence both in specific biosensors and in strains with an inactivated receptor protein gene (rhlR or lasR), as well as in E. coli strain MG1655/pXen7 with constitutive transcription of the lux operon, i.e. these compounds directly affected the luciferase reaction.