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Abstract <p>Cryo-ET (cryo-electron tomography) is a powerful tool for studying the structure of biological objects in their native state. However, Cryo-ET is still not widely used to study cellular organelles including cell nucleus and chromatin in situ. In our work, we aimed to investigate the possibilities of employing cryo-ET for studying the architecture of natively preserved chromatin, focusing on the possibility of using a cryoCLEM (cryo-correlative light and electron microscopy) approach to target final steps of sample preparation by focused ion beam (FIB) milling exactly to the area of interest, namely, heterochromatin loci.</p>

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Analysis of Heteroсhromatin Mesoscale Architecture by Cryo-Electron Tomography

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • E. P. Kazakov,
  • Y. M. Chesnokov,
  • I. I. Kireev,
  • S. A. Golyshev

摘要

Abstract

Cryo-ET (cryo-electron tomography) is a powerful tool for studying the structure of biological objects in their native state. However, Cryo-ET is still not widely used to study cellular organelles including cell nucleus and chromatin in situ. In our work, we aimed to investigate the possibilities of employing cryo-ET for studying the architecture of natively preserved chromatin, focusing on the possibility of using a cryoCLEM (cryo-correlative light and electron microscopy) approach to target final steps of sample preparation by focused ion beam (FIB) milling exactly to the area of interest, namely, heterochromatin loci.