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Abstract <p>The compactness of biomolecules largely determines their functionality. It has previously been shown that the compactness of DNA and RNA can be estimated from the relative intensity of the low-frequency (LF) region of Raman scattering (RS) spectra; however, the applicability of this approach to complexes and mixtures of DNA with other biomolecules remained uncertain due to the unknown relative contribution of these molecules to the LF Raman spectrum. In this work, the experimental Raman spectra of DNA, protein, and fatty acid are compared, and it is shown that the intensity of the LF region is significantly higher for native DNA than for the other two types of biomolecules. Additionally, it is demonstrated that upon complex formation between DNA and protein, the shape of the Raman spectrum of the complex is close to that of the DNA spectrum, while the relative intensity of the LF region of the spectrum decreases. This indicates the dominance of the RS signal from DNA and an increase in DNA compactness during complex formation. The obtained results contribute to the development of a method for assessing the compactness of various biomolecules based on the analysis of LF RS spectra and its application to complex biomolecular systems, such as cell nuclei.</p>

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Relative Intensity of the Low-Frequency Raman Spectra of Nucleic Acids, Proteins, and Lipids

  • 期刊论文

  • 发表日期：

    2026年2月1日

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  • O. D. Parashchuk,
  • A. Yu. Sosorev,
  • A. A. Trubitsyn,
  • T. S. Mustafin,
  • I. V. Chicherin,
  • U. E. Piunova,
  • D. Yu. Parashchuk

摘要

Abstract

The compactness of biomolecules largely determines their functionality. It has previously been shown that the compactness of DNA and RNA can be estimated from the relative intensity of the low-frequency (LF) region of Raman scattering (RS) spectra; however, the applicability of this approach to complexes and mixtures of DNA with other biomolecules remained uncertain due to the unknown relative contribution of these molecules to the LF Raman spectrum. In this work, the experimental Raman spectra of DNA, protein, and fatty acid are compared, and it is shown that the intensity of the LF region is significantly higher for native DNA than for the other two types of biomolecules. Additionally, it is demonstrated that upon complex formation between DNA and protein, the shape of the Raman spectrum of the complex is close to that of the DNA spectrum, while the relative intensity of the LF region of the spectrum decreases. This indicates the dominance of the RS signal from DNA and an increase in DNA compactness during complex formation. The obtained results contribute to the development of a method for assessing the compactness of various biomolecules based on the analysis of LF RS spectra and its application to complex biomolecular systems, such as cell nuclei.