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<p>Imprinted genes play a key role in regulating mammalian placental and embryonic development. Here, we generated glutaminyl-peptide cyclotransferase-knockout (<i>Qpct</i><sup>−/−</sup>) mice utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) platform and identified <i>Qpct</i> as a novel anti-angiogenic factor in regulating mouse placentation. Compared with <i>Qpct</i><sup>+/+</sup> mice, placentae and embryos (<i>Qpct</i><sup>−/+</sup> and <i>Qpct</i><sup>−/−</sup>) showed significant overgrowth at embryonic Day 12.5 (E12.5), E15.5, and E18.5. Using single-cell transcriptome analysis of 32 309 cells from <i>Qpct</i><sup>+/+</sup> and <i>Qpct</i><sup>−/−</sup> mouse placentae, we identified 13 cell clusters via single-nucleus RNA sequencing (snRNA-seq) (8880 <i>Qpct</i><sup>+/+</sup> and 13 577 <i>Qpct</i><sup>−/−</sup> cells) and 20 cell clusters via single-cell RNA sequencing (scRNA-seq) (6567 <i>Qpct</i><sup>+/+</sup> and 3285 <i>Qpct</i><sup>−/−</sup> cells). Furthermore, we observed a global up-regulation of pro-angiogenic genes in the <i>Qpct</i><sup>−/−</sup> background. Immunohistochemistry assays revealed a notable increase in the number of blood vessels in the decidual and labyrinthine layers of E15.5 <i>Qpct</i><sup>−/+</sup> and <i>Qpct</i><sup>−/−</sup> mice. Moreover, the elevation of multiple pairs of ligand-receptor interactions was observed in decidual cells, endothelial cells, and macrophages, promoting angiogenesis and inflammatory response. Our findings indicate that loss of maternal <i>Qpct</i> leads to altered phenotypic characteristics of placentae and embryos and promotes angiogenesis in murine placentae.</p>

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Single-cell transcriptome analysis reveals abnormal angiogenesis and placentation by loss of imprinted glutaminyl-peptide cyclotransferase

  • 期刊论文

  • 发表日期：

    2025年6月27日

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  • Jing Guo,
  • Jihong Zheng,
  • Ruixia Li,
  • Jindong Yao,
  • He Zhang,
  • Xu Wang,
  • Chao Zhang

摘要

Imprinted genes play a key role in regulating mammalian placental and embryonic development. Here, we generated glutaminyl-peptide cyclotransferase-knockout (Qpct−/−) mice utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) platform and identified Qpct as a novel anti-angiogenic factor in regulating mouse placentation. Compared with Qpct+/+ mice, placentae and embryos (Qpct−/+ and Qpct−/−) showed significant overgrowth at embryonic Day 12.5 (E12.5), E15.5, and E18.5. Using single-cell transcriptome analysis of 32 309 cells from Qpct+/+ and Qpct−/− mouse placentae, we identified 13 cell clusters via single-nucleus RNA sequencing (snRNA-seq) (8880 Qpct+/+ and 13 577 Qpct−/− cells) and 20 cell clusters via single-cell RNA sequencing (scRNA-seq) (6567 Qpct+/+ and 3285 Qpct−/− cells). Furthermore, we observed a global up-regulation of pro-angiogenic genes in the Qpct−/− background. Immunohistochemistry assays revealed a notable increase in the number of blood vessels in the decidual and labyrinthine layers of E15.5 Qpct−/+ and Qpct−/− mice. Moreover, the elevation of multiple pairs of ligand-receptor interactions was observed in decidual cells, endothelial cells, and macrophages, promoting angiogenesis and inflammatory response. Our findings indicate that loss of maternal Qpct leads to altered phenotypic characteristics of placentae and embryos and promotes angiogenesis in murine placentae.