Scholarlink

<p>Reversible electroporation (RE) involves applying pulsed electric fields to briefly disrupt cell membrane channels, allowing molecular transfer while preserving cell viability. Advances in RE combined with micro- and nano-devices have improved efficiency and safety in cellular analysis and engineering, holding substantial promise for biological research and precision medicine. This review summarizes progress in RE technology, spanning underlying theoretical principles, practical implementation, and emerging applications. By integrating mechanistic insights with parameter assessment, we strengthen process understanding of RE to support further optimization. To clarify RE implementation strategies, we present advances in micro- and nano-devices based on varying electric field control approaches. Using these platforms, we examine improvements in intracellular analysis, cellular engineering, drug delivery, and cell sampling to illustrate state-of-the-art RE applications. Finally, we outline future directions and trends for RE systems aimed at molecular mechanism mapping and personalized precision medicine, emphasizing their increasing relevance in practical settings.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Reversible electroporation: controllably opening the cellular doorway

  • 期刊论文

  • 发表日期：

    2026年5月1日

查看全文
  • Yuhao Zhou,
  • Zhihui Zhang,
  • Feng Liu,
  • Xinran Jiang,
  • Chaojuan Yang,
  • Yang Wang,
  • Zaizai Dong,
  • Lingqian Chang

摘要

Reversible electroporation (RE) involves applying pulsed electric fields to briefly disrupt cell membrane channels, allowing molecular transfer while preserving cell viability. Advances in RE combined with micro- and nano-devices have improved efficiency and safety in cellular analysis and engineering, holding substantial promise for biological research and precision medicine. This review summarizes progress in RE technology, spanning underlying theoretical principles, practical implementation, and emerging applications. By integrating mechanistic insights with parameter assessment, we strengthen process understanding of RE to support further optimization. To clarify RE implementation strategies, we present advances in micro- and nano-devices based on varying electric field control approaches. Using these platforms, we examine improvements in intracellular analysis, cellular engineering, drug delivery, and cell sampling to illustrate state-of-the-art RE applications. Finally, we outline future directions and trends for RE systems aimed at molecular mechanism mapping and personalized precision medicine, emphasizing their increasing relevance in practical settings.