Abstract <p>Conducting polymers offer attractive opportunities for flexible electronic devices when their electrical properties can be directionally controlled. Here, we report a scalable approach to fabricate in-plane anisotropic poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) thin films by combining spin coating with wet strain. The electromechanical coupling behavior of the films was systematically examined in dry and ethylene glycol (EG)-wetted states to identify suitable strain treatment conditions. While dry films largely followed the resistance changes expected from geometric deformation, EG-wetted films exhibited a pronounced negative electromechanical response at low strain. Analysis of apparent resistivity trends revealed a distinct minimum at approximately 25% strain, suggesting a condition of strain-induced structural reorganization. PEDOT:PSS films treated under these conditions displayed clear in-plane electrical anisotropy with lower sheet resistance along the strain direction. This wet-strain processing strategy provides a practical route to introduce electrical anisotropy into PEDOT:PSS films, while maintaining compatibility with established device architectures on flexible substrates.</p> Graphical abstract <p></p>