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<p>The algaroba (<i>Prosopis juliflora</i>) is an invasive tree species with promising potential as a biomass source for biofuel production. This study evaluated the feasibility of using algaroba wood bark to produce fermentable sugars. The biomass underwent chemical pretreatment with sodium dithionite and was hydrolyzed using <i>Trichoderma reesei</i> ATCC 26921 cellulase (10&#xa0;FPU&#xa0;gds<sup>−1</sup>). Hydrolysates were analyzed for fermentable sugar content. Pretreatment proved effective, achieving a 75% reduction in lignin (from 28.06 to 6.84%). During enzymatic hydrolysis, untreated bark yielded 3.07&#xa0;mg&#xa0;mL<sup>−1</sup> (12.93%), while pretreated bark yielded 2.90&#xa0;mg&#xa0;mL<sup>−1</sup> (11.38%) of reducing sugars after 24&#xa0;h—both considered low relative to the biomass’s high holocellulose content. The results indicate that pretreatment successfully removed lignin and preserved cellulose structure. However, to improve enzymatic conversion of cellulose to glucose, further optimization is required, including adjustments in enzyme loading, solids concentration, or incubation time.</p> Graphical abstract <p></p>

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Evaluation of the application of algaroba wood as a feedstock to obtain fermentable sugars for bioethanol production

  • 期刊论文

  • 发表日期：

    2026年1月5日

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  • Yuri Uriel Cerqueira Gil Braz Moreira,
  • Geise Camila de Araújo Ribeiro,
  • Carine Tondo Alves,
  • Lilian Lefol Nani Guarieiro

摘要

The algaroba (Prosopis juliflora) is an invasive tree species with promising potential as a biomass source for biofuel production. This study evaluated the feasibility of using algaroba wood bark to produce fermentable sugars. The biomass underwent chemical pretreatment with sodium dithionite and was hydrolyzed using Trichoderma reesei ATCC 26921 cellulase (10 FPU gds−1). Hydrolysates were analyzed for fermentable sugar content. Pretreatment proved effective, achieving a 75% reduction in lignin (from 28.06 to 6.84%). During enzymatic hydrolysis, untreated bark yielded 3.07 mg mL−1 (12.93%), while pretreated bark yielded 2.90 mg mL−1 (11.38%) of reducing sugars after 24 h—both considered low relative to the biomass’s high holocellulose content. The results indicate that pretreatment successfully removed lignin and preserved cellulose structure. However, to improve enzymatic conversion of cellulose to glucose, further optimization is required, including adjustments in enzyme loading, solids concentration, or incubation time.

Graphical abstract