Abstract <p>Diabetes diagnoses traditionally rely on testing levels of glucose in the blood. These tests are uncomfortable, inconvenient, and pose infection risks. Urine glucose detection is a safer and cheaper alternative. This research developed a colorimetric urine glucose sensor using glucose oxidase (GOx) immobilized on a chitosan-Fe²⁺ complex. The system worked by GOx catalyzing glucose to produce hydrogen peroxide, which oxidized Fe²⁺ to Fe³⁺. The Fe³⁺-SCN⁻ complex generated measurable absorbance and visible color changes proportional to glucose concentration. Chitosan's metal-chelating properties and functional groups enable stable enzyme immobilization and operational stability; creating a sensitive and selective glucose detection system.</p> Graphical Abstract <p></p>