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<p>Bone remodeling and immune function are dynamically regulated through cell–cell and cell–matrix interactions by stem and mature cell populations. We investigated the hypothesis that monocytes and pre-osteoblasts respond to cyclic tensile stress and paracrine interactions by differentiating into macrophage-like and osteoblast-like cells. 20% cyclic equibiaxial strain was applied to monocytic U937 and pre-osteoblastic ST2 cells for 72&#xa0;h. Increased levels of CD11B, CD14, IL-6, and IL-8 in U937 indicated monocytic differentiation. Increased ALP expression and calcium deposition in ST2 indicated differentiation towards osteoblastic lineage. Overall, application of cyclic strain and pre-osteoblastic co-culture induced differentiation in this cyclically strained bone model.</p> Graphical abstract <p></p>

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Co-stimulation with equibiaxial strain and pre-osteoblast co-culture differentiates monocytes in a bone model

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月17日

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  • Maria R. Ward Rashidi,
  • Catherine S. Snyder,
  • Kathleen M. Burkhard,
  • Raneem Ahmad,
  • Isha Bhorkar,
  • Geeta Mehta

摘要

Bone remodeling and immune function are dynamically regulated through cell–cell and cell–matrix interactions by stem and mature cell populations. We investigated the hypothesis that monocytes and pre-osteoblasts respond to cyclic tensile stress and paracrine interactions by differentiating into macrophage-like and osteoblast-like cells. 20% cyclic equibiaxial strain was applied to monocytic U937 and pre-osteoblastic ST2 cells for 72 h. Increased levels of CD11B, CD14, IL-6, and IL-8 in U937 indicated monocytic differentiation. Increased ALP expression and calcium deposition in ST2 indicated differentiation towards osteoblastic lineage. Overall, application of cyclic strain and pre-osteoblastic co-culture induced differentiation in this cyclically strained bone model.

Graphical abstract