<p>This research aimed to develop a biocomposite containing bioactive glass nanoparticles (BGs) for skin wound healing. BGs, synthesized based on the SiO₂-CaO-B₂O₃-ZnO system, had amorphous nature, spherical-shaped morphology and nano-sized particles. Composite sponges were fabricated by the freeze-drying and characterized. Among them, the sponge with 5 wt% of BGs (CA-G5) exhibited excellent water absorption ability, high porosity (88%) and interconnected pores with an average pore size of 130&#xa0;µm suitable for migration and proliferation of fibroblasts. Moreover, CA-G5 showed improved tensile strength and elastic modulus in both dry and wet states and antibacterial activity against S. aureus and E. coli bacteria. Biological assays confirmed the positive role of BGs on human dermal fibroblast cell viability, proliferation and attachment. The <i>in vitro</i> biomineralization assay in simulated wound fluid showed apatite deposition after 7&#xa0;days of immersion. This study suggests the developed biocomposite presents great potential for wound healing applications.</p> Graphical abstract <p>Schematic representation of developing a biocomposite composed of Chitosan, Alginate and Zinc and Boron-releasing bioactive glass nanoparticles for wound healing applications. The resulting biocomposite exhibited promising mechanical properties, water absorption ability, and antibacterial activity against S. aureus and E. coli, and supported human dermal fibroblast viability and attachment</p>