Chemical synthesis and biosynthesis Ag-doped CoFe₂O₄ nanomaterial for biomedical application

Nanomaterials with highly attractive magnetic properties are recently of great interest for biomedical applications. In this study, the Ag-doped CoFe₂O₄ nanoparticles were successfully synthesized and revealed significant antibacterial activities using P. aeruginosa, E. coli, and S. aureus as tested species. Among that, the CoFe_1.8Ag_0.2O₄ (CFA0.2) showed the best antibacterial activity with their corresponding inhibition zone diameter of 18, 20, and 19 mm for P. aeruginosa, E. coli, and S. aureus, respectively. Moreover, the cytotoxic activity against HepG2 human cancer cell line, IC₅₀ values, was examined for the biosynthesized CFA0.2 (CFA0.2PC), and showed a decrease to 68.9 µg/mL from the value of 77.3 µg/mL of CFA0.2. Especially, the hyperthermia testing showed a significant increase in the specific absorption rate of the CFA0.2PC compared with that of CoFe₂O₄ and CFA0.2, and these characteristics were well consistent with the theoretical analysis. These results confirmed the high-potential biomaterial applications of Ag-doped CoFe₂O₄ nanoparticles.