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<p>IgM-selective proteolytic cleavage can become a groundbreaking tool for immunoassay-based bioanalysis. We tested the utility of a newly introduced IgM-selective protease in three different bioanalytical applications and showed that protease treatment can eliminate rheumatoid factor interference and simplify IgM isotyping. Surprisingly, we discovered that a gene therapy-induced immune response was underestimated in an anti-capsid IgG assay when treatment-emergent IgM were present in the matrix. Selective IgM digestion eliminated this interference.</p> Graphical Abstract <p></p>

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A Tool to Eliminate IgM Immunoassay Interference

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月11日

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  • Alexander Pöhler,
  • Uwe Wessels,
  • Roland F. Staack,
  • Maria Nordgren,
  • Rolf Lood,
  • Kay-Gunnar Stubenrauch

摘要

IgM-selective proteolytic cleavage can become a groundbreaking tool for immunoassay-based bioanalysis. We tested the utility of a newly introduced IgM-selective protease in three different bioanalytical applications and showed that protease treatment can eliminate rheumatoid factor interference and simplify IgM isotyping. Surprisingly, we discovered that a gene therapy-induced immune response was underestimated in an anti-capsid IgG assay when treatment-emergent IgM were present in the matrix. Selective IgM digestion eliminated this interference.

Graphical Abstract