Scholarlink

<p>Preclinical imaging is crucial in biomedical research for non-invasive monitoring of various parameters in live animals over time, providing vital information while minimizing animal use. Optical imaging, the most commonly employed preclinical tool, offers ease of use, cost-effective instrumentation, and diverse applications. However, these advantages complicate the rational design, execution, and analysis of optical imaging experiments. This review highlights the specific conditions, strengths, and weaknesses of bioluminescence and fluorescence imaging. We discuss critical parameters essential for optimizing imaging settings to ensure accurate experimental outcomes. Additionally, we provide guidelines and address intrinsic limitations to assist scientists in making informed decisions and avoiding potential data shortcomings.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optical imaging in biomedical research: guidelines and practical insights

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月27日

查看全文
  • Tianjiao Chu,
  • Xiaoqi Zheng,
  • Bella B. Manshian,
  • Stefaan J. Soenen

摘要

Preclinical imaging is crucial in biomedical research for non-invasive monitoring of various parameters in live animals over time, providing vital information while minimizing animal use. Optical imaging, the most commonly employed preclinical tool, offers ease of use, cost-effective instrumentation, and diverse applications. However, these advantages complicate the rational design, execution, and analysis of optical imaging experiments. This review highlights the specific conditions, strengths, and weaknesses of bioluminescence and fluorescence imaging. We discuss critical parameters essential for optimizing imaging settings to ensure accurate experimental outcomes. Additionally, we provide guidelines and address intrinsic limitations to assist scientists in making informed decisions and avoiding potential data shortcomings.