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<p>This study characterized drug resistance and virulence in <i>Enterococcus faecalis</i> and <i>Enterococcus faecium</i> isolated from pet dogs and cats in Guangdong Province (Guangzhou, Shenzhen, Dongguan, and Foshan) and Shanghai, China. It investigated potential cross-host transmission and genetic diversity associated with enterococci carrying oxazolidinone resistance genes. Bacterial isolation, antimicrobial susceptibility and virulence phenotype testing, detection of resistance and virulence genes, and whole-genome sequencing&#xa0;were performed. Enterococci isolated from pets exhibited resistance to linezolid and tedizolid, harboring the <i>cfr</i>(D), <i>optrA</i>, and <i>poxtA</i> resistance genes. A strain of <i>E. faecium</i> harbored a plasmid carrying both the <i>cfr</i>(D) and <i>optrA</i> genes. Compared to <i>E. faecalis</i>, <i>E. faecium</i> exhibited a higher hemolysis rate but a lower biofilm formation rate. Thirteen virulence genes were detected in <i>E. faecalis</i>; detection rates for <i>gelE</i>, <i>sprE</i>, <i>agg</i>, and <i>fsr</i> series genes decreased annually, while rates for the hemolysin <i>cyl</i> series, <i>asa1</i>, and <i>efaA</i> genes increased. Genomic analysis revealed clonal transmission of <i>optrA</i>- and <i>poxtA</i>-positive <i>E. faecium</i> within pets. Genetic environment analysis implicated IS<i>1216</i> as a key facilitator in the dissemination of oxazolidinone resistance genes. These findings demonstrated that pet dogs and cats harbored enterococci carrying oxazolidinone resistance genes, with these strains exhibiting clonal transmission among companion animals.</p>

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Detection and characterization of oxazolidinone resistance genes in enterococci from companion animals

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月28日

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  • Jianxin Hu,
  • Jinyu Yang,
  • Kaixin Zhang,
  • Yongxiang Zhang,
  • Lingxiang Mao,
  • Sujuan Wu,
  • Honghao Huang,
  • Yafei Li,
  • Dongping Zeng,
  • Yanjun Dong,
  • Wenguang Xiong,
  • Zhenling Zeng

摘要

This study characterized drug resistance and virulence in Enterococcus faecalis and Enterococcus faecium isolated from pet dogs and cats in Guangdong Province (Guangzhou, Shenzhen, Dongguan, and Foshan) and Shanghai, China. It investigated potential cross-host transmission and genetic diversity associated with enterococci carrying oxazolidinone resistance genes. Bacterial isolation, antimicrobial susceptibility and virulence phenotype testing, detection of resistance and virulence genes, and whole-genome sequencing were performed. Enterococci isolated from pets exhibited resistance to linezolid and tedizolid, harboring the cfr(D), optrA, and poxtA resistance genes. A strain of E. faecium harbored a plasmid carrying both the cfr(D) and optrA genes. Compared to E. faecalis, E. faecium exhibited a higher hemolysis rate but a lower biofilm formation rate. Thirteen virulence genes were detected in E. faecalis; detection rates for gelE, sprE, agg, and fsr series genes decreased annually, while rates for the hemolysin cyl series, asa1, and efaA genes increased. Genomic analysis revealed clonal transmission of optrA- and poxtA-positive E. faecium within pets. Genetic environment analysis implicated IS1216 as a key facilitator in the dissemination of oxazolidinone resistance genes. These findings demonstrated that pet dogs and cats harbored enterococci carrying oxazolidinone resistance genes, with these strains exhibiting clonal transmission among companion animals.