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<p>The emergence of carbapenem-resistant <i>Enterobacterales</i> (CRE) poses a critical threat to global health security. Veterinary hospitals may serve as potential hotspots for antimicrobial resistance (AMR) gene dissemination, yet surveillance in these environments remains limited. This study was carried out to screen the spread of CRE in the environment of a veterinary hospital. Totally, we collected 283 environmental swab samples from a veterinary hospital in Guangzhou, China. The samples were screened for meropenem-resistant isolates, and polymerase chain reaction amplification was performed to detect <i>bla</i><sub>NDM</sub> genes. Species identification was performed using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Two isolates representing previously unreported <i>bla</i><sub>NDM</sub>-positive species were selected for whole-genome sequencing. Thirty-two meropenem-resistant isolates were recovered, with 15 isolates (5.3%) testing positive for <i>bla</i><sub>NDM</sub> genes. All the <i>bla</i><sub>NDM</sub>-positive isolates belonged to <i>Enterobacterales</i>, including one isolate each of <i>Pseudescherichia vulneris</i> and <i>Pantoea dispersa</i>, representing the first reported <i>bla</i><sub>NDM</sub>-positive strains of these species. Whole-genome sequencing of these two isolates revealed that both harbored <i>bla</i><sub>NDM-5</sub> genes on IncX3 plasmids with conjugation frequencies of 7.5 × 10⁻<sup>5</sup> and 6.0 × 10⁻<sup>5</sup>, respectively. The <i>P. dispersa</i> strain exhibited high genetic similarity (core-genome single nucleotide polymorphisms = 12) to isolates of <i>Oryza sativa</i> origin from India lacking <i>bla</i><sub>NDM</sub> genes, suggesting recent plasmid acquisition. The detection of <i>bla</i><sub>NDM-5</sub> in these previously unreported host species is concerning given their exceptional environmental persistence and stress tolerance. These findings highlight the urgent need for enhanced One Health surveillance approaches encompassing veterinary environments as critical components of AMR networks.</p>

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Emergence of blaNDM-5-carrying Pseudescherichia vulneris and Pantoea dispersa isolated from the veterinary hospital environment

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月30日

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  • Zhongpeng Cai,
  • Weihang Liu,
  • Xinger Wen,
  • Bingqing Zhu,
  • Luchao Lv,
  • Zhiwen Wu,
  • Chanping Zhi

摘要

The emergence of carbapenem-resistant Enterobacterales (CRE) poses a critical threat to global health security. Veterinary hospitals may serve as potential hotspots for antimicrobial resistance (AMR) gene dissemination, yet surveillance in these environments remains limited. This study was carried out to screen the spread of CRE in the environment of a veterinary hospital. Totally, we collected 283 environmental swab samples from a veterinary hospital in Guangzhou, China. The samples were screened for meropenem-resistant isolates, and polymerase chain reaction amplification was performed to detect blaNDM genes. Species identification was performed using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Two isolates representing previously unreported blaNDM-positive species were selected for whole-genome sequencing. Thirty-two meropenem-resistant isolates were recovered, with 15 isolates (5.3%) testing positive for blaNDM genes. All the blaNDM-positive isolates belonged to Enterobacterales, including one isolate each of Pseudescherichia vulneris and Pantoea dispersa, representing the first reported blaNDM-positive strains of these species. Whole-genome sequencing of these two isolates revealed that both harbored blaNDM-5 genes on IncX3 plasmids with conjugation frequencies of 7.5 × 10⁻5 and 6.0 × 10⁻5, respectively. The P. dispersa strain exhibited high genetic similarity (core-genome single nucleotide polymorphisms = 12) to isolates of Oryza sativa origin from India lacking blaNDM genes, suggesting recent plasmid acquisition. The detection of blaNDM-5 in these previously unreported host species is concerning given their exceptional environmental persistence and stress tolerance. These findings highlight the urgent need for enhanced One Health surveillance approaches encompassing veterinary environments as critical components of AMR networks.