Background <p>Tiger stripes, parallel bands within regurgitant Doppler spectra, are often ascribed to oscillating intracardiac structures. Their frequency and clinical relevance are unclear.</p> Methods <p>We retrospectively reviewed 4,567 transthoracic echocardiograms (TTEs) in adults between 18 and 65 years of age for tiger stripes on pulsed-wave (PW)/continuous-wave (CW) Doppler and correlated with valve pathology, presence of oscillating masses, and clinical findings.</p> Results <p>Tiger stripes occurred in 5.4% of studies (95% CI 4.8–6.1%), predominantly with mitral and aortic regurgitation jets (MR/AR) and high-velocity tricuspid regurgitation (TR) jets. Stripes were not seen when oscillating masses were present, and were more conspicuous in mild–moderate jets and higher-velocity Doppler spectra. Audible Doppler cooing sound was common; no association with clinical musical murmurs.</p> Conclusions <p>Tiger stripes may represent a benign spectral Doppler artifact. In isolation, they should not prompt additional imaging for oscillating masses or be used to grade regurgitation severity.</p>