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<p>Pseudorabies virus (PRV,&#xa0;<i>SuidAlphaherpesvirus</i> 1) causes substantial economic losses in swine production. Here, we report the development of DNA aptamers targeting the PRV glycoprotein D (gD) through an optimized SELEX protocol. After 15 selection cycles, Apt-gD-2 demonstrated nanomolar affinity (Kd = 6.107 ± 0.476&#xa0;nM) and high specificity for gD, as validated by an enzyme-linked aptamer-sorbent assay (ELASA) and fluorescence microscopy. Molecular docking revealed hydrogen bonding as the key interaction mechanism. The developed ic-ELASA achieved 83.3% concordance with qPCR in clinical samples, supporting its utility for on-farm PRV surveillance. These findings highlight the potential of aptamer-based diagnostic methods for rapid, sensitive, and onsite detection of PRV, offering a promising tool for disease control in the swine industry.</p>

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DNA aptamers targeting glycoprotein D enable specific detection of pseudorabies virus (PRV)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月7日

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  • Zhihao Wang,
  • Yan Qiao,
  • Jiafu Zhao,
  • Xiaotian Chang,
  • Heshui Zhu,
  • Chao Zhang

摘要

Pseudorabies virus (PRV, SuidAlphaherpesvirus 1) causes substantial economic losses in swine production. Here, we report the development of DNA aptamers targeting the PRV glycoprotein D (gD) through an optimized SELEX protocol. After 15 selection cycles, Apt-gD-2 demonstrated nanomolar affinity (Kd = 6.107 ± 0.476 nM) and high specificity for gD, as validated by an enzyme-linked aptamer-sorbent assay (ELASA) and fluorescence microscopy. Molecular docking revealed hydrogen bonding as the key interaction mechanism. The developed ic-ELASA achieved 83.3% concordance with qPCR in clinical samples, supporting its utility for on-farm PRV surveillance. These findings highlight the potential of aptamer-based diagnostic methods for rapid, sensitive, and onsite detection of PRV, offering a promising tool for disease control in the swine industry.