Scholarlink

<p>In plant, lycopene β-cyclase is a crucial enzyme in carotenoid metabolic pathway, which can induce color alteration. Carrots (<i>Daucus carota</i>) possess two genes encoding lycopene β-cyclase, namely <i>DcLcyB1</i> and <i>DcLcyB2</i>. Little is known regarding the functional disparities between these two proteins in regulating carrot carotenoid accumulation. We found that the expression level of <i>DcLcyB2</i> was higher than that of <i>DcLcyB1</i> in carrot roots. Enzyme reaction in <i>E. coli</i> demonstrated that both two DcLcyB proteins were capable of cycloconverting lycopene to β-carotene, but DcLcyB2 tended to have a higher preference for monocyclic carotene substrates, resulting in more α-carotene production. After the <i>DcLcyBs</i> were separately overexpressed in red carrots, the roots turned yellow, accompanied by the reduction of lycopene and β-carotene content and the entire carotenoid metabolism flowed downstream towards xanthophylls. The expression levels of <i>DcCHXE</i>, <i>DcCYP97A3</i>, <i>DcCHXB1</i> and <i>DcCHXB2</i> in the <i>DcLcyB-OE</i> lines raised sharply. After gene editing of DcLcyB1/2, the α-/β-carotene ratio changed conspicuously, particularly in the <i>dclcyb2</i> mutants, where α-carotene content dropped sharply, while β-carotene remained high. The expression levels of most structural genes in carotenoid pathway responded dynamically. Our results enriched the understanding of functionally redundant but differentiated roles of two DcLcyB isoenzymes in carrot root coloring.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

DcLcyB1 and DcLcyB2, two lycopene β-cyclases with partial functional overlap modulate carotene profiles in carrot roots via distinct catalytic properties

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月1日

查看全文
  • Ya-Hui Wang,
  • Pei-Zhuo Liu,
  • Rong-Rong Zhang,
  • Yu-Qing Zhang,
  • Hui-Ru Wang,
  • Yu-Jie Sun,
  • Jing Ma,
  • Zhi-Sheng Xu,
  • Ai-Sheng Xiong

摘要

In plant, lycopene β-cyclase is a crucial enzyme in carotenoid metabolic pathway, which can induce color alteration. Carrots (Daucus carota) possess two genes encoding lycopene β-cyclase, namely DcLcyB1 and DcLcyB2. Little is known regarding the functional disparities between these two proteins in regulating carrot carotenoid accumulation. We found that the expression level of DcLcyB2 was higher than that of DcLcyB1 in carrot roots. Enzyme reaction in E. coli demonstrated that both two DcLcyB proteins were capable of cycloconverting lycopene to β-carotene, but DcLcyB2 tended to have a higher preference for monocyclic carotene substrates, resulting in more α-carotene production. After the DcLcyBs were separately overexpressed in red carrots, the roots turned yellow, accompanied by the reduction of lycopene and β-carotene content and the entire carotenoid metabolism flowed downstream towards xanthophylls. The expression levels of DcCHXE, DcCYP97A3, DcCHXB1 and DcCHXB2 in the DcLcyB-OE lines raised sharply. After gene editing of DcLcyB1/2, the α-/β-carotene ratio changed conspicuously, particularly in the dclcyb2 mutants, where α-carotene content dropped sharply, while β-carotene remained high. The expression levels of most structural genes in carotenoid pathway responded dynamically. Our results enriched the understanding of functionally redundant but differentiated roles of two DcLcyB isoenzymes in carrot root coloring.