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<p>The high-quality chromosomal-level genome assembly (627.72&#xa0;Mb, contig N50 1.80&#xa0;Mb) of <i>Uncaria rhynchophylla</i>, a Chinese herb used for hypertension and Alzheimer's disease. Integrated genomic and transcriptomic analyses identified 46,909 genes, including 72 <i>UrWRKY</i> transcription factors. Sixty-four candidate genes for monoterpenoid indole alkaloid (MIA) biosynthesis were screened. Functional validation confirmed UrTDC6 catalyzes tryptamine formation and UrLAMT1/UrLAMT2 catalyze loganin production. Correlation analysis linked MIA content with <i>UrWRKY</i> expression, identifying <i>UrWRKY37</i> as a key regulator. UrWRKY37 directly activates <i>UrTDC</i> and <i>UrSGD</i> transcription. Overexpression in hairy roots upregulated MIA pathway genes and significantly increased accumulation of isocorynoxeine, corynoxeine, and isorhynchophylline. This work elucidates MIA biosynthesis and its transcriptional regulation in <i>U. rhynchophylla</i>.</p>

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The genome of the medicinal plant Uncaria rhynchophylla provides new insights into monoterpenoid indole alkaloid metabolism and its molecular regulatory mechanism

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月3日

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  • Detian Mu,
  • Lingyun Wan,
  • Yingying Shao,
  • Limei Pan,
  • Xinghui Liu,
  • Iain W. Wilson,
  • Zhixing Qing,
  • Yu Zhou,
  • Ying Lu,
  • Yingjie He,
  • Lina Zhu,
  • Jine Fu,
  • Shugen Wei,
  • Xiaojun Ma,
  • Chi Song,
  • Deyou Qiu,
  • Qi Tang

摘要

The high-quality chromosomal-level genome assembly (627.72 Mb, contig N50 1.80 Mb) of Uncaria rhynchophylla, a Chinese herb used for hypertension and Alzheimer's disease. Integrated genomic and transcriptomic analyses identified 46,909 genes, including 72 UrWRKY transcription factors. Sixty-four candidate genes for monoterpenoid indole alkaloid (MIA) biosynthesis were screened. Functional validation confirmed UrTDC6 catalyzes tryptamine formation and UrLAMT1/UrLAMT2 catalyze loganin production. Correlation analysis linked MIA content with UrWRKY expression, identifying UrWRKY37 as a key regulator. UrWRKY37 directly activates UrTDC and UrSGD transcription. Overexpression in hairy roots upregulated MIA pathway genes and significantly increased accumulation of isocorynoxeine, corynoxeine, and isorhynchophylline. This work elucidates MIA biosynthesis and its transcriptional regulation in U. rhynchophylla.