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<p>The S-RNase-based self-incompatibility locus (<i>S</i>-locus) in <i>Petunia</i> species contains 16–20 <i>F-box</i> genes, which collaboratively function in the recognition and subsequent degradation of non-self S-RNases, while distinguishing them from self S-RNase. However, the number of <i>S</i>-locus<i> F</i>-<i>box</i> genes (<i>SFBB</i>s) physically interacted with non-self S-RNases remains uncertain in <i>Pyrus</i> species. Utilizing Pacbio long-read sequencing, we successfully assembled the genome of pear cultivar ‘Yali’ (<i>Pyrus bretschneideri</i>), and identified 19 <i>SFBB</i>s from the <i>Pyrus S</i><sub><i>17</i></sub>-locus spanning approximately 1.78 Mb. Additionally, we identified 17–21 <i>SFBB</i>s from other <i>Pyrus</i> and <i>Malus S</i>-loci spanning a range of 1.35 to 2.64 Mb. Based on the phylogenetic analysis, it was determined that <i>Pyrus</i> and <i>Malus SFBB</i>s could be classified into 22 groups, denoted as I to XXII. At amino acid level, <i>SFBB</i>s within a given group exhibited average identities ranged from 88.9% to 97.9%. Notably, all 19 <i>SFBB</i>s from the <i>S</i><sub><i>17</i></sub>-locus co-segregated with <i>S</i><sub><i>17</i></sub><i>-RNase</i>, with 18 of them being specifically expressed in pollen. Consequently, these 18 pollen-specifically expressed <i>SFBB</i>s are considered potential candidates for the pollen-<i>S</i> determinant. Intriguingly, out of the 18 pollen-specifically expressed <i>SFBB</i>s, eight demonstrated interactions with at least one non-self S-RNase, while the remaining SFBBs failed to recognize any S-RNase. These findings provide compelling evidence supporting the existence of a collaborative non-self-recognition system governing self-incompatibility in pear species.</p>

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Long-read genome sequencing reveals the sequence characteristics of pear self-incompatibility locus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月1日

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  • Chao Gu,
  • Ying Xu,
  • Lei Wu,
  • Xueping Wang,
  • Kaijie Qi,
  • Xin Qiao,
  • Zewen Wang,
  • Qionghou Li,
  • Min He,
  • Shaoling Zhang

摘要

The S-RNase-based self-incompatibility locus (S-locus) in Petunia species contains 16–20 F-box genes, which collaboratively function in the recognition and subsequent degradation of non-self S-RNases, while distinguishing them from self S-RNase. However, the number of S-locus F-box genes (SFBBs) physically interacted with non-self S-RNases remains uncertain in Pyrus species. Utilizing Pacbio long-read sequencing, we successfully assembled the genome of pear cultivar ‘Yali’ (Pyrus bretschneideri), and identified 19 SFBBs from the Pyrus S17-locus spanning approximately 1.78 Mb. Additionally, we identified 17–21 SFBBs from other Pyrus and Malus S-loci spanning a range of 1.35 to 2.64 Mb. Based on the phylogenetic analysis, it was determined that Pyrus and Malus SFBBs could be classified into 22 groups, denoted as I to XXII. At amino acid level, SFBBs within a given group exhibited average identities ranged from 88.9% to 97.9%. Notably, all 19 SFBBs from the S17-locus co-segregated with S17-RNase, with 18 of them being specifically expressed in pollen. Consequently, these 18 pollen-specifically expressed SFBBs are considered potential candidates for the pollen-S determinant. Intriguingly, out of the 18 pollen-specifically expressed SFBBs, eight demonstrated interactions with at least one non-self S-RNase, while the remaining SFBBs failed to recognize any S-RNase. These findings provide compelling evidence supporting the existence of a collaborative non-self-recognition system governing self-incompatibility in pear species.