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Background <p>Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease that presents as a symmetrical polyarthritis characterized by joint pain, swelling, and stiffness. This study aimed to evaluate the expression of microRNA-24-3p (miRNA-24-3p), in the plasma of RA patients and compare it with that of healthy controls with the goal of establishing a specific expression profile for early diagnosis and improved patient care.</p> Methodology <p>This case-control study was carried out on 100 RA patients attending Rehabilitation and Physical Medicine department and 50 age and sex-matched control individuals. Disease severity score (DAS-28) was used to assess the disease’s level. Real-time polymerase chain reaction (qPCR) was used for detection of plasma level of miRNA-24-3p and other lab tests including blood picture, c-reactive protein (CRP), erythrocytes sedimentation rate (ESR) and anti-cyclic citrullinated peptide (anti-CCP) were also performed.</p> Results <p>The miRNA-24-3p expression was statistically significant higher among RA cases than controls (<i>p</i> &lt; 0.001) and was positively associated with higher ESR, white blood cells (WBCs) and along with the number of tendered/swallow joints (<i>p</i> &lt; 0.05). Higher CRP, ESR, Anti-CCP, and miRNA-24-3p expression were found to be significant predictors of DAS28 scores in univariable analysis.</p> <p>Higher CRP and miRNA-24-3p expression exhibit substantial impact in multivariate analysis, indicating a strong statistical significance in their association with higher DAS28 grades.</p> Conclusion <p>The elevated expression of miR-24-3p suggests its probable contribution to both the onset of RA and the activity measured by DAS28 score. Consequently, assessing its expression levels may serve as a valuable tool for the diagnosis and management of RA.</p>

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Plasma levels of microRNA-24-3p as a potential diagnostic biomarker for rheumatoid arthritis in some Egyptian patients

  • 期刊论文

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  • 发表日期：

    2026年3月12日

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  • Ibrahim Mohammed Rageh,
  • Ahmed Mamdouh Awadallah,
  • Noha Hosni Ibrahim,
  • Rana Gamal Abd-Elnasser,
  • Seham Gouda Ameen

摘要

Background

Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease that presents as a symmetrical polyarthritis characterized by joint pain, swelling, and stiffness. This study aimed to evaluate the expression of microRNA-24-3p (miRNA-24-3p), in the plasma of RA patients and compare it with that of healthy controls with the goal of establishing a specific expression profile for early diagnosis and improved patient care.

Methodology

This case-control study was carried out on 100 RA patients attending Rehabilitation and Physical Medicine department and 50 age and sex-matched control individuals. Disease severity score (DAS-28) was used to assess the disease’s level. Real-time polymerase chain reaction (qPCR) was used for detection of plasma level of miRNA-24-3p and other lab tests including blood picture, c-reactive protein (CRP), erythrocytes sedimentation rate (ESR) and anti-cyclic citrullinated peptide (anti-CCP) were also performed.

Results

The miRNA-24-3p expression was statistically significant higher among RA cases than controls (p < 0.001) and was positively associated with higher ESR, white blood cells (WBCs) and along with the number of tendered/swallow joints (p < 0.05). Higher CRP, ESR, Anti-CCP, and miRNA-24-3p expression were found to be significant predictors of DAS28 scores in univariable analysis.

Higher CRP and miRNA-24-3p expression exhibit substantial impact in multivariate analysis, indicating a strong statistical significance in their association with higher DAS28 grades.

Conclusion

The elevated expression of miR-24-3p suggests its probable contribution to both the onset of RA and the activity measured by DAS28 score. Consequently, assessing its expression levels may serve as a valuable tool for the diagnosis and management of RA.