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Background <p>Circular RNAs are stable non-coding RNAs that regulate biological processes via sponging microRNAs, interacting with RNA binding proteins, as well as controlling gene expression. In ulcerative colitis (UC), hsa_circ_0004104 may worsen inflammation and disrupt immune responses by affecting the JAK/STAT pathway and compromising epithelial barrier integrity.</p> Subjects and methods <p>The present study included 50 patients with UC and 25 healthy subjects as controls. The relative expression of hsa_circ_0004104 was analyzed using real-time quantitative polymerase chain reaction (qPCR), while the concentration of circulating Janus kinase 2 was determined using enzyme-linked immunosorbent assay (ELISA).</p> Results <p>hsa_circ_0004104 was significantly upregulated in active and inactive UC in contrast to control group. In addition, the active UC patients gave a significantly elevated JAK2 level in contrast to inactive and control groups. Furthermore, there was a potent positive correlation relating hsa_circ_0004104 to JAK2 expression in active UC patients.</p> Conclusion <p>Higher levels of hsa_circ_0004104 and JAK2 expression in active UC patients suggest their role in disease activity and their potential use as therapeutic targets and diagnostic biomarkers for UC management.</p>

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Circular ribonucleic acid 0004104 as a potential biomarker and its relation to Janus kinase 2 in ulcerative colitis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月4日

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  • Roaa Tarek Elbannan,
  • Hannan Kamal Abdelaziz,
  • Eman Ahmed Shaat,
  • AM Ibrahim,
  • Hend A. yassin

摘要

Background

Circular RNAs are stable non-coding RNAs that regulate biological processes via sponging microRNAs, interacting with RNA binding proteins, as well as controlling gene expression. In ulcerative colitis (UC), hsa_circ_0004104 may worsen inflammation and disrupt immune responses by affecting the JAK/STAT pathway and compromising epithelial barrier integrity.

Subjects and methods

The present study included 50 patients with UC and 25 healthy subjects as controls. The relative expression of hsa_circ_0004104 was analyzed using real-time quantitative polymerase chain reaction (qPCR), while the concentration of circulating Janus kinase 2 was determined using enzyme-linked immunosorbent assay (ELISA).

Results

hsa_circ_0004104 was significantly upregulated in active and inactive UC in contrast to control group. In addition, the active UC patients gave a significantly elevated JAK2 level in contrast to inactive and control groups. Furthermore, there was a potent positive correlation relating hsa_circ_0004104 to JAK2 expression in active UC patients.

Conclusion

Higher levels of hsa_circ_0004104 and JAK2 expression in active UC patients suggest their role in disease activity and their potential use as therapeutic targets and diagnostic biomarkers for UC management.