Scholarlink

Background <p>Brucellosis, an endemic zoonotic infection in Egypt, presents a significant public health concern. This study aimed to identify <i>Brucella</i> isolates from animal and human samples collected from six governorates using serological, bacteriological, and molecular methods. The susceptibility of the isolates to selected antibiotics was also evaluated.</p> Results <p>Serological testing revealed positive brucellosis in 86.8% of human and 4.7% animal sera. 54 suspected <i>Brucella</i> isolates were recovered from human and animal samples. <i>Brucella melitensis</i> was the predominant species followed by <i>Brucella abortus</i>, which were molecularly confirmed using (<i>bcsp31</i>). The isolates harbored key virulence genes of <i>Brucella</i> (<i>bvfA</i>, <i>virB</i>, and <i>ure</i>) and the RNA polymerase-<i>rpoB</i> gene. The isolates expressed overall susceptibility to standard antibiotics used to treat brucellosis, while exhibiting resistance to ceftriaxone, cefotaxime, rifampicin, tetracycline, and linezolid.</p> Conclusions <p>Serological testing proved to be the most practical and rapid detection method for large-scale screening in developing countries, while molecular analysis identified <i>Brucella melitensis</i> to be the predominant species, with the presence of key virulence genes in some isolates. Traditionally prescribed antibiotics remain effective against brucellosis; however, special care should be taken due to the emergence of resistant isolates. Tigecycline and linezolid showed that they can serve as potential alternatives in combination treatments. This study provides a broader, more comprehensive multi-governorate perspective on brucellosis in Egypt offering insights for future surveillance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Serological, molecular, and antimicrobial susceptibility surveillance of Brucella species in Egypt

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月1日

查看全文
  • Mayada Hammam,
  • Reham Samir,
  • Inass El-Gaafarawi,
  • Khalid Abou-Gazia,
  • Marwa ElRakaiby

摘要

Background

Brucellosis, an endemic zoonotic infection in Egypt, presents a significant public health concern. This study aimed to identify Brucella isolates from animal and human samples collected from six governorates using serological, bacteriological, and molecular methods. The susceptibility of the isolates to selected antibiotics was also evaluated.

Results

Serological testing revealed positive brucellosis in 86.8% of human and 4.7% animal sera. 54 suspected Brucella isolates were recovered from human and animal samples. Brucella melitensis was the predominant species followed by Brucella abortus, which were molecularly confirmed using (bcsp31). The isolates harbored key virulence genes of Brucella (bvfA, virB, and ure) and the RNA polymerase-rpoB gene. The isolates expressed overall susceptibility to standard antibiotics used to treat brucellosis, while exhibiting resistance to ceftriaxone, cefotaxime, rifampicin, tetracycline, and linezolid.

Conclusions

Serological testing proved to be the most practical and rapid detection method for large-scale screening in developing countries, while molecular analysis identified Brucella melitensis to be the predominant species, with the presence of key virulence genes in some isolates. Traditionally prescribed antibiotics remain effective against brucellosis; however, special care should be taken due to the emergence of resistant isolates. Tigecycline and linezolid showed that they can serve as potential alternatives in combination treatments. This study provides a broader, more comprehensive multi-governorate perspective on brucellosis in Egypt offering insights for future surveillance.