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Background <p>Diabetes mellitus is a disordered syndrome which has destructive effects on cells. The aim of this study is to reduce the side effects caused by glucose using treating cells with hydroalcoholic extract of <i>Prosopis farcta</i> in electrospun 3D culture with poly lactic acid (PLA).</p> Materials and methods <p>The extract was prepared by maceration method, and the plant compounds were identified by (GC–MS). Human adipose-derived mesenchymal stem cells <b>(</b>hADMSCs) was treated with different concentrations of extract, and the level of cytotoxicity was evaluated by MTT test. Then, the cells were cultured in hyperglycemic medium with concentrations of 25, 50 and 75&#xa0;mM and hyperglycemic medium treated with optimal concentration of extract (200&#xa0;μg/ml) for 24, 48 and 72&#xa0;h. The expression of antioxidant genes SOD1 and HO1 was investigated by RT-PCR method. The scratch test was performed to investigate cell migration and JC-1 to measure the mitochondrial membrane potential, and the TUNEL test was performed to investigate DNA fragmentation.</p> Results <p>The most compounds of the extract were (9‐Octadecenoic acid (E)/heptadecene-(8)/carbonic acid-(1), oleic acid, and Z, E‐2,13‐Octadecadien‐1‐ol). The extract was able to increase the survival and proliferation of cells that were reduced in the hyperglycemic environment. It also decreased the expression levels of <i>SOD1</i> and <i>HO1</i> genes. In the scratch test, the rate of proliferation and migration of cells increased significantly in 48 and 72&#xa0;h. DNA fragmentation decreased in the cells treated with the extract, but the mitochondrial membrane potential increased significantly.</p> Conclusion <p>Due to its abundant antioxidant compounds, <i>Prosopis farcta</i> was able to reduce the harmful effects of glucose by modulating the expression of antioxidant genes, reducing apoptosis, increasing the rate of cell growth and migration, and mitochondrial membrane potential.</p>

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Antidiabetic effect of Prosopis farcta extract on human adipose-derived mesenchymal stem cells in 3D culture electrospun with PLA

  • 期刊论文

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  • 发表日期：

    2025年6月3日

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  • Nasrin Heidari,
  • Faezeh Namvar,
  • Mina Ramezani,
  • Zeinab Piravar

摘要

Background

Diabetes mellitus is a disordered syndrome which has destructive effects on cells. The aim of this study is to reduce the side effects caused by glucose using treating cells with hydroalcoholic extract of Prosopis farcta in electrospun 3D culture with poly lactic acid (PLA).

Materials and methods

The extract was prepared by maceration method, and the plant compounds were identified by (GC–MS). Human adipose-derived mesenchymal stem cells (hADMSCs) was treated with different concentrations of extract, and the level of cytotoxicity was evaluated by MTT test. Then, the cells were cultured in hyperglycemic medium with concentrations of 25, 50 and 75 mM and hyperglycemic medium treated with optimal concentration of extract (200 μg/ml) for 24, 48 and 72 h. The expression of antioxidant genes SOD1 and HO1 was investigated by RT-PCR method. The scratch test was performed to investigate cell migration and JC-1 to measure the mitochondrial membrane potential, and the TUNEL test was performed to investigate DNA fragmentation.

Results

The most compounds of the extract were (9‐Octadecenoic acid (E)/heptadecene-(8)/carbonic acid-(1), oleic acid, and Z, E‐2,13‐Octadecadien‐1‐ol). The extract was able to increase the survival and proliferation of cells that were reduced in the hyperglycemic environment. It also decreased the expression levels of SOD1 and HO1 genes. In the scratch test, the rate of proliferation and migration of cells increased significantly in 48 and 72 h. DNA fragmentation decreased in the cells treated with the extract, but the mitochondrial membrane potential increased significantly.

Conclusion

Due to its abundant antioxidant compounds, Prosopis farcta was able to reduce the harmful effects of glucose by modulating the expression of antioxidant genes, reducing apoptosis, increasing the rate of cell growth and migration, and mitochondrial membrane potential.