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Background <p>The chronic inflammatory and immunological disease "Asthma" is characterized by oxidative stress disruptions and is typically treated with glucocorticoids, the most potent anti-inflammatory agents. However, prolonged use may result in many adverse effects. Earthworms have antioxidant, anti-inflammatory, and anti-fibrotic characteristics. This study investigates the possible protective properties of Allolobophora caliginosa extract (AcE) in asthma model.</p> Method <p>Twenty-four female mice were allocated into four groups: control, ovalbumin (OVA), OVA + AcE (45&#xa0;mg/kg), and OVA + dexamethasone (Dexa) (1&#xa0;mg/kg). We assessed bronchoalveolar lavage fluid (BALF) inflammatory cells, nasal scratching frequency, BALF and serum IgE, ILs-4, -1<i>β</i>, and -13, and OVA-specific IgE levels, pulmonary oxidative stress markers (malondialdehyde (MDA) and nitric oxide (NO) levels, reduced glutathione (GSH) concentration and catalase (CAT) and glutathione S-transferase (GST) activity), the frequency of CD19<sup>+</sup> B-lymphocytes, histopathology (H&amp;E and Masson’s trichrome stains), and the immunohistochemistry expression of p-nuclear factor Kappa-B (NF-κB) p65 in the lungs.</p> Results <p>AcE markedly reduced BALF inflammatory cells, nasal scratching frequency, IgE, OVA-specific IgE, IL-4, -1<i>β</i>, -13, CD19<sup>+</sup> B cells, MDA, NO levels, as well as asthmatic mice histological inflammatory scores. The histologic and histochemical studies showed improved lung structure, decreased goblet cell hyperplasia, and reduced collagen precipitation after AcE treatment.</p> Conclusion <p>The anti-asthmatic properties of AcE are attributed to its anti-inflammatory and antioxidant properties, specifically its capacity to inhibit NF-κB and CD19<sup>+</sup> expression and inhibit oxidative stress.</p>

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Anti-inflammatory and antioxidant effects of Allolobophora caliginosa extract against Ovalbumin-induced airway inflammation in asthmatic female BALB/c mice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月25日

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  • Islam Ahmed Abdelmawgood,
  • Tarek Atia,
  • Abeer Mahmoud Badr,
  • Ayman Saber Mohamed,
  • Mennatallah H. Abdelaziz,
  • Noha Ahmed Mahana,
  • Hader Ibrahim Sakr,
  • Reham S. Al-Fakharany,
  • Tamer D. Abdelaziz,
  • Ahmed A. Damanhory,
  • Adel Alghamdi,
  • Marwa Ahmed Abdelfattah,
  • Hadeer Hesham Abdelfattah

摘要

Background

The chronic inflammatory and immunological disease "Asthma" is characterized by oxidative stress disruptions and is typically treated with glucocorticoids, the most potent anti-inflammatory agents. However, prolonged use may result in many adverse effects. Earthworms have antioxidant, anti-inflammatory, and anti-fibrotic characteristics. This study investigates the possible protective properties of Allolobophora caliginosa extract (AcE) in asthma model.

Method

Twenty-four female mice were allocated into four groups: control, ovalbumin (OVA), OVA + AcE (45 mg/kg), and OVA + dexamethasone (Dexa) (1 mg/kg). We assessed bronchoalveolar lavage fluid (BALF) inflammatory cells, nasal scratching frequency, BALF and serum IgE, ILs-4, -1β, and -13, and OVA-specific IgE levels, pulmonary oxidative stress markers (malondialdehyde (MDA) and nitric oxide (NO) levels, reduced glutathione (GSH) concentration and catalase (CAT) and glutathione S-transferase (GST) activity), the frequency of CD19+ B-lymphocytes, histopathology (H&E and Masson’s trichrome stains), and the immunohistochemistry expression of p-nuclear factor Kappa-B (NF-κB) p65 in the lungs.

Results

AcE markedly reduced BALF inflammatory cells, nasal scratching frequency, IgE, OVA-specific IgE, IL-4, -1β, -13, CD19+ B cells, MDA, NO levels, as well as asthmatic mice histological inflammatory scores. The histologic and histochemical studies showed improved lung structure, decreased goblet cell hyperplasia, and reduced collagen precipitation after AcE treatment.

Conclusion

The anti-asthmatic properties of AcE are attributed to its anti-inflammatory and antioxidant properties, specifically its capacity to inhibit NF-κB and CD19+ expression and inhibit oxidative stress.