Background <p><i>Chrysomya bezziana</i> (Villeneuve, 1914), the screw-worm fly, is a significant parasitic blow fly species that causes obligatory myiasis in livestock and, in some cases, in humans. Its ability to infest wounds and cause extensive tissue damage makes it a significant concern in the veterinary and public health sectors. Accurate and timely identification of <i>C. bezziana</i> is critical for managing outbreaks and implementing control measures. This study confirms the occurrence of <i>C. bezziana</i> in Egypt through both morphological and molecular identification, as only a few studies have reported its presence in Egypt so far. Adult samples of <i>C. bezziana</i> were collected using meat-baited traps situated in the cemeteries of two localities in Egypt (Port Said and El-Sharkia Governorates). DNA was extracted from the specimens for molecular identification using the cytochrome oxidase I (<i>cox1</i>) gene.</p> Results <p>Morphologically, <i>C. bezziana</i> adult flies were identified by a row of hairs on the dorsal surface of the wing's stem vein, blackish anterior spiracles, and the most distinctive characteristic, a white calypter with a whitish-yellow distal end, which differentiates this species from the most closely related species, <i>Chrysomya megacephala</i>. Genetically, it was found that based on a 703 bp fragment of the <i>cox1</i> gene, <i>C. bezziana</i> was accurately identified, and further phylogenetic analysis confirmed the DNA-based identification of adult specimens of <i>C. bezziana</i> examined. This finding confirms the presence of <i>C. bezziana</i> and expands our knowledge of its distribution in Egypt.</p> Conclusion <p>This study is the first to report the occurrence of <i>C. bezziana</i> in Egypt using an integration of molecular and morphological methods.</p>