Silencing circ-RNA-049637 influences hydatid outer cyst wall formation by liver fibrosis

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2024年6月10日

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Baheti Kalifu,
Yuan Meng,
Zhi-Gang Ma,
Chao Ma,
Guang-Lei Tian,
Jin-Guo Wang,
Xiong Chen

摘要

Introduction and objectives

In diseases characterized by fibrosis, hepatic stellate cells (HSCs) are mesenchymal cells that play an important role in liver fibrosis. circRNAs are involved in regulating hydatid exocyst formation through miRNA sponge adsorption. The mechanisms of hepatic cystic hydatid outer cyst formation, HSC, and liver fibrosis are unclear.

Materials and methods

Based on our sequencing data, we validated the mechanism by which circRNA-049637 regulated hepatic cystic hydatid growth and promoted outer fibrocystic wall formation.

Results

Our results revealed that circRNA_049637 silencing promoted the proliferation of LX-2 human HSCs, affected the cell cycle, and increased the mRNA and protein expression levels of liver fibrosis-related indicators such as α-SMA, COL1A1, COL3A1, and TGFβRII.

Conclusions

CircRNA_049637 may induce the formation of hepatic hydatid cysts by promoting hepatic fibrosis via HSC activation.