Background <p>Different pathologies can affect the optic pathway structures in children, and early detection of these pathologies requires knowledge of their normal measurements. This study aimed to establish a standardized method for measuring the optic pathway structures and report their normal values in children up to six years of age using brain MRI.</p> Methods <p>We retrospectively analyzed the brain MR images of pediatric patients from infants to six years of age; scans that were interpreted as normal were included in the study. Axial T2-weighted images were used for optic nerve (intra-orbital, pre-chiasmatic) and optic tract measurements. Coronal T2-weighted images were used for optic chiasm measurements. Patients were stratified into six age groups for analysis.</p> Results <p>A total of 104 MRI studies of the brain were included; there were 65 males and 39 females. The mean age of all the patients was 26 ± 12&#xa0;months. An increase in diameter was observed with age for all measurements. There is a strong positive correlation between age and mean diameter of each of the intra-orbital optic nerve (<i>r</i> = 0.670), pre-chiasmatic optic nerve (<i>r</i> = 0.703), optic tract (<i>r</i> = 0.693), as well as the height of the optic chiasm (<i>r</i> = 0.716).</p> Conclusions <p>Using axial and coronal brain MRI, we suggested standardized locations for measuring the optic pathway structures and reported their normal values in children from birth to 6&#xa0;years of age.</p>