Scholarlink

Background <p>The sirtuin (SIRT) family has a crucial role in aging biology. SIRT1 is a NAD<sup>+</sup>-dependent deacetylase. SIRT2 is an NAD-dependent deacetylase that mediates deacetylation of alpha-tubulin. SIRT3 is a mitochondrial enzyme that regulates mitochondrial functions, including ATP synthesis, reactive oxygen species production, fatty acid oxidation, ketone metabolism, and cellular death. SIRT4 is an ADP-ribosyltransferase that downregulates glutamate dehydrogenase in β cells, with a subsequent reduction in insulin secretion. SIRT5 regulates post-translational modifications. SIRT6 is a mono-ADP-ribosyl transferase. SIRT7 is present in the nucleoli and controls RNA polymerase I. The SIRT1 gene is present on chromosome 10 at the 10q21.3 locus.</p> Methods <p>This study included 2 groups. Group 1 included 35 elderly patients with type 2 diabetes mellitus (T2DM). Group 2 included 35 elderly non-diabetic subjects. Laboratory investigations included complete blood picture and kidney and liver function tests. Lipid profile, fasting blood sugar (FBS), two-hour postprandial blood sugar (2-h-PPS), and hemoglobin A1c (HbA1c) were measured. SIRT1 protein and mRNA expressions were measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR), respectively.</p> Results <p>No significant differences were detected between the two groups concerning hemoglobin (Hb) level, total leukocytic count, or platelet count (<i>P</i> = 0.064, 0.251, and 0.333, respectively). However, FBS, 2-h-PPS, and HbA1c were significantly higher in cases compared to controls (<i>P</i> &lt; 0.001 for each). Additionally, total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) were significantly higher in cases compared to controls (P &lt; 0.001, 0.001, and 0.005, respectively). However, there was no significant difference in high-density lipoprotein cholesterol (HDL-C) (<i>P</i> = 0.314). SIRT1 mRNA and protein expressions were significantly higher in cases compared to controls (<i>P</i><b> &lt; </b>0.001, for both). SIRT1 had significant negative correlations with body mass index (BMI) (r = − 0.316, <i>P</i> = 0.006), duration of DM (r = − 0.308, <i>P</i> = 0.009), FBS (r = − 0.581, <i>P</i> &lt; 0.001), 2-h-PPS (r = − 0.676, <i>P</i> &lt; 0.001), HbA1c (r = − 0.648, <i>P</i> &lt; 0.001), TC <b>(</b>r = − 0.232, <i>P</i> = 0.029), and LDL-C (r = − 0.263, <i>P</i> = 0.013).</p> Conclusion <p>SIRT1 mRNA and protein expressions were significantly upregulated in T2DM. SIRT1 expression had significant negative correlations with BMI, duration of DM, FBS, 2-h-PPS, HbA1c, TC, and LDL-C. BMI and HbA1c are determinants of SIRT1 expression in elderly patients with T2DM, as confirmed by regression analysis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Sirtuin 1 expression in elderly patients with type 2 diabetes mellitus

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月20日

查看全文
  • Mohamed M. M. Hassaan,
  • Imam M. Esmayel,
  • Mayada M. Mousa,
  • Samia Hussein,
  • Ahmed K. Y. Alkolaly,
  • Batoul A. Hegazy,
  • Rodalia M. Makhlouf

摘要

Background

The sirtuin (SIRT) family has a crucial role in aging biology. SIRT1 is a NAD+-dependent deacetylase. SIRT2 is an NAD-dependent deacetylase that mediates deacetylation of alpha-tubulin. SIRT3 is a mitochondrial enzyme that regulates mitochondrial functions, including ATP synthesis, reactive oxygen species production, fatty acid oxidation, ketone metabolism, and cellular death. SIRT4 is an ADP-ribosyltransferase that downregulates glutamate dehydrogenase in β cells, with a subsequent reduction in insulin secretion. SIRT5 regulates post-translational modifications. SIRT6 is a mono-ADP-ribosyl transferase. SIRT7 is present in the nucleoli and controls RNA polymerase I. The SIRT1 gene is present on chromosome 10 at the 10q21.3 locus.

Methods

This study included 2 groups. Group 1 included 35 elderly patients with type 2 diabetes mellitus (T2DM). Group 2 included 35 elderly non-diabetic subjects. Laboratory investigations included complete blood picture and kidney and liver function tests. Lipid profile, fasting blood sugar (FBS), two-hour postprandial blood sugar (2-h-PPS), and hemoglobin A1c (HbA1c) were measured. SIRT1 protein and mRNA expressions were measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR), respectively.

Results

No significant differences were detected between the two groups concerning hemoglobin (Hb) level, total leukocytic count, or platelet count (P = 0.064, 0.251, and 0.333, respectively). However, FBS, 2-h-PPS, and HbA1c were significantly higher in cases compared to controls (P < 0.001 for each). Additionally, total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) were significantly higher in cases compared to controls (P < 0.001, 0.001, and 0.005, respectively). However, there was no significant difference in high-density lipoprotein cholesterol (HDL-C) (P = 0.314). SIRT1 mRNA and protein expressions were significantly higher in cases compared to controls (P < 0.001, for both). SIRT1 had significant negative correlations with body mass index (BMI) (r = − 0.316, P = 0.006), duration of DM (r = − 0.308, P = 0.009), FBS (r = − 0.581, P < 0.001), 2-h-PPS (r = − 0.676, P < 0.001), HbA1c (r = − 0.648, P < 0.001), TC (r = − 0.232, P = 0.029), and LDL-C (r = − 0.263, P = 0.013).

Conclusion

SIRT1 mRNA and protein expressions were significantly upregulated in T2DM. SIRT1 expression had significant negative correlations with BMI, duration of DM, FBS, 2-h-PPS, HbA1c, TC, and LDL-C. BMI and HbA1c are determinants of SIRT1 expression in elderly patients with T2DM, as confirmed by regression analysis.