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<p>Agricultural industries search for biotechnological alternatives for waste management as they mean a significant concern and environmental challenge. More importantly, within a circular economy concept, such secondary substrates can be used to produce value-added compounds. This work is aimed at obtaining bioactive compounds from citrus waste by using a homemade enzymatic cocktail from <i>Aspergillus niger</i> LBM 134. The fungal enzymes were produced using raw sugarcane bagasse as substrate, which increased the ecological sustainability and the cost-effectiveness of the bioprocess. As the most relevant enzyme of this cocktail, a β-glucosidase showed to optimally act at 50&#xa0;°C, retaining up to 70% of residual activity after 72&#xa0;h. By means of an optimized enzyme-assisted extraction, the crude enzymatic cocktail produced was efficiently employed to extract the phenolics hesperetin, quinic, <i>p</i>-coumaric, and gallic acid, and the bioactive amino acid tryptophan from citrus waste. These assays yielded approximately 112% and 30% of phenolic compounds over alkaline conventional and commercial enzyme extraction methods, respectively.</p> Graphical Abstract <p></p>

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Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月11日

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  • Gabriela Verónica Díaz,
  • Romina Olga Coniglio,
  • Laura Ester Ortellado,
  • Pedro Darío Zapata,
  • María Alejandra Martínez,
  • María Isabel Fonseca

摘要

Agricultural industries search for biotechnological alternatives for waste management as they mean a significant concern and environmental challenge. More importantly, within a circular economy concept, such secondary substrates can be used to produce value-added compounds. This work is aimed at obtaining bioactive compounds from citrus waste by using a homemade enzymatic cocktail from Aspergillus niger LBM 134. The fungal enzymes were produced using raw sugarcane bagasse as substrate, which increased the ecological sustainability and the cost-effectiveness of the bioprocess. As the most relevant enzyme of this cocktail, a β-glucosidase showed to optimally act at 50 °C, retaining up to 70% of residual activity after 72 h. By means of an optimized enzyme-assisted extraction, the crude enzymatic cocktail produced was efficiently employed to extract the phenolics hesperetin, quinic, p-coumaric, and gallic acid, and the bioactive amino acid tryptophan from citrus waste. These assays yielded approximately 112% and 30% of phenolic compounds over alkaline conventional and commercial enzyme extraction methods, respectively.

Graphical Abstract