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<p>Wheat blast, caused by the <i>Magnaporthe oryzae Triticum</i> pathotype (MoT), is a devastating fungal disease that poses a significant global threat to wheat production. Rapid diagnosis is crucial for effective monitoring, containment, and management of this highly transmissible pathogen. In this study, we developed an integrated field-detection kit, named <Emphasis Type="Underline">W</Emphasis>heat <Emphasis Type="Underline">B</Emphasis>last <Emphasis Type="Underline">R</Emphasis>apid <Emphasis Type="Underline">D</Emphasis>etection <Emphasis Type="Underline">Kit</Emphasis> (WB-RD kit), which utilizes recombinase polymerase amplification coupled with a nucleic acid lateral flow immunoassay (RPA–NALFIA). The kit is stable for room temperature transportation and achieves a limit of detection as low as 0.1&#xa0;ng/μL for MoT genomic DNA. The RPA reaction only requires a constant temperature of 39°C for 10&#xa0;min. The positive result is indicated by the appearance of both a control line and a test line on the lateral flow strip within 5&#xa0;min. This rapid, precise, user-friendly, and portable method is suitable for both on‑site diagnosis and phytosanitary inspection of seed shipments, providing a practical tool for frontline disease monitoring and quarantine.</p>

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A simple and rapid method for wheat blast detection in the field

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月28日

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  • Ye Peng,
  • Jianglin Xu,
  • Batiseba Tembo,
  • Xinyao He,
  • Pawan Kumar Singh,
  • Guo-Liang Wang,
  • Houxiang Kang

摘要

Wheat blast, caused by the Magnaporthe oryzae Triticum pathotype (MoT), is a devastating fungal disease that poses a significant global threat to wheat production. Rapid diagnosis is crucial for effective monitoring, containment, and management of this highly transmissible pathogen. In this study, we developed an integrated field-detection kit, named Wheat Blast Rapid Detection Kit (WB-RD kit), which utilizes recombinase polymerase amplification coupled with a nucleic acid lateral flow immunoassay (RPA–NALFIA). The kit is stable for room temperature transportation and achieves a limit of detection as low as 0.1 ng/μL for MoT genomic DNA. The RPA reaction only requires a constant temperature of 39°C for 10 min. The positive result is indicated by the appearance of both a control line and a test line on the lateral flow strip within 5 min. This rapid, precise, user-friendly, and portable method is suitable for both on‑site diagnosis and phytosanitary inspection of seed shipments, providing a practical tool for frontline disease monitoring and quarantine.